br Data Xylogenesis a process of wood fiber formation

Data Xylogenesis (a process of wood fiber formation) is often characterized by two distinct stages: “early” and “late”. The first stage involves undifferentiated precursor pteryxin that are characterized by their primary cell wall, whereas the later stage includes a succession of events including secondary cell wall deposition and programmed cell death leading to full cellular differentiation into tracheary elements [4]. In this study we aimed to elucidate the differences in proteome composition between those two stages. By means of mass spectrometry analysis we identified significant enrichment in proteins related to cellular energy together with protein and primary cell wall metabolism in undifferentiated samples, whereas differentiated wood fibers were exhibiting peptides involved in cell wall polysaccharide biosynthesis.
Experimental design, materials and methods
Acknowledgments The authors would like to acknowledge the financial assistance provided by Stillinger Forest Science Research and Dr. Bob Stillinger scholarships. We are grateful to Drs. Allan Caplan and Slawomir A. Dziedzic for their critical review of the manuscript. We acknowledge Drs. Lee Deobald and Andrzej Paszczynski for their technical assistance in LC-MS/MS. We acknowledge the M.J. Murdock Charitable Trust for their support in the purchase of the Q-TOF Premier mass spectrometer. Authors also would like to thank the PRIDE team for help with data upload to the PRIDE database.
Data
Experimental design, material and methods To profile differentially glycosylated serum proteins between disease conditions, each serum sample was subjected to parallel pulldown using 20 different lectins, prior to on-bead tryptic digest and LC-MS analysis (Fig. 1). The lectins used are: AAL, BPL, ConA, DSA, ECA, EPHA, GNL, HAA, HPA, JAC, LPHA, MAA, NPL, PSA, SBA, SNA, STL, UEA, WFA and WGA [2].
Acknowledgments
Data
Experimental design, materials and methods
Acknowledgments This research was supported by Grants from the National Institutes of Health/National Cancer InstituteR03CA169959 and the Feist-Weiller Cancer Center, LSU Health Shreveport.
Value of the data
Data Endogenous dopamine D4 receptor (DRD4) gene expression in SH-SY5Y cells was detected by RT-PCR using cDNA derived from the cell line (Fig. 1). To test whether the polymorphisms within the promoter change the promoter activity, luciferase activity was measured under the influence of the DRD4 promoter into which polymorphisms were introduced (Fig. 2 and Table 1). All of the reporter plasmids containing the DRD4 fragment exhibited significantly higher luciferase activity than the control pGL3-Basic, and although every possible combination of haplotypes was investigated, there were no activity differences among the introduced mutations in SH-SY5Y cells (Figs. 3 and 4).
Experimental design, materials and methods
Acknowledgements
Specifications table
Value of the data
Data The ACTOne CB1 cell line was optimized for experimental conditions related to 96 well plate reader detection of cAMP driven fluorescence. Cells were probed for optimal forskolin stimulation of cAMP driven fluorescence, the concentration of CB1 agonist CP 55,940 needed to suppress cAMP driven fluorescence, as well as the time point for most stable fluorescent signal. Furthermore, the robustness of the ACTOne CB1 cells was evaluated with respect to cell passage number and the ability of the cell line to reveal Gs coupled responses following pertussis toxin Gi/o inactivation.
Experimental design, materials and methods
Acknowledgements Dr. Jimmy Lu from Codex Biosolutions provided both the ACTOne cell lines and the support to get the assay started. Funding for Chaela Presley was supplied by the University of Tennessee Neuroscience Institute and College of Pharmacy. Funding for Ammaar Abidi was supplied by the University of Tennessee College of Dentistry.