Archives
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The strand domain swap in the UbV
2020-01-21
The β strand domain swap in the UbV.XR dimer appears to be a unique arrangement for a Ub-based dimer. In solution, free Ub has a KD of ∼5 mM for the monomer-dimer equilibrium, and the two subunits in the dimer adopt a range of relative orientations involving residues from Ub’s β sheet (Liu et al., 2
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Additionally we analysed the features of
2020-01-21
Additionally, we analysed the features of apomict and sexual Boechera species DNMT proteins. All DNMT proteins showed highly similar theoretical pI, molecular weight and predicted sub-cellular localization (Table 2). Discussion Identification of the structure and expression levels of DNMTs durin
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As the first allotetraploid fish in
2020-01-21
As the first allotetraploid fish in the world, our lab selectively breed the allotetraploid hybrid fish (4nAT, 4n = 200) from the distant hybridization offspring F3 of female Red Crucian Carp (Carassius auratus red var., abbreviated as RCC, 2n = 100) and male Common Carp (Cyprinius carpio L., abbrev
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The absence of the LIG
2020-01-21
The absence of the LIG3 gene in yeasts has prevented the use of genetically tractable lower eukaryotes, such as Saccharomyces cerevisiae and Schizosaccharomyces pombe, as models to gain insights into the cellular functions of and interplay between the DNA ligases encoded by the LIG1, LIG3 and LIG4 g
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In the present study we combined activity
2020-01-21
In the present study, we combined activity-based protein profiling, biochemical and genetic approaches to identify the PLCPs involved in the sphingolipid pathway leading to plant PCD. By using Arabidopsis VU 0364439 and leaves, we demonstrate that this sub-class of plant proteases is activated in r
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For in vitro expression analysis of CXCL and CXCRs by
2020-01-21
For in vitro expression analysis of CXCL8 and CXCRs by stimulated leucocytes, peripheral blood leucocytes (PBLs) were isolated using Percoll density gradient centrifugation as described earlier . Cell suspensions were adjusted to 5×10/ml. Four milliliters of tissue culture medium were plated per wel
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The pharmacokinetics properties of compound were evaluated
2020-01-21
The pharmacokinetics properties of compound were evaluated in male Sprague Dawley rats following IV (0.7mg/kg) and oral (2.0mg/kg) dosing. The total body clearance and the terminal half-life were 0.73 L/h/kg and 5.1h, respectively. After oral administration the compound showed good bioavailability (
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Numerous small molecule CRF receptor antagonists have
2020-01-21
Numerous small molecule CRF receptor antagonists have been reported in the literature. Some representative examples are shown in . In preclinical studies (DMP696), , was found to be efficacious in preclinical behavioral models for anxiety and depression. In an open label clinical trial with (R121919
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Assessment of the activity of native and
2020-01-21
Assessment of the activity of native and denatured TAT-CPG2 after penetration suggests that TAT-CPG2 has been successfully delivered into the cells, and the transduced enzyme is functional inside the cells. The enzyme activity of both native and denatured TAT-fusion proteins in the cultured HepG2 Ga
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Magnetic interactions between metal ions are usually
2020-01-21
Magnetic interactions between metal ions are usually described by superexchange via intermediate ligands. Although the general principles of the superexchange mechanism are essentially the same for d and f ions, calculations of exchange parameters for lanthanides are more difficult than for transiti
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The proliferation of CCA cell lines can
2020-01-21
The proliferation of CCA cell lines can be stimulated by estrogen and other growth factors (Alvaro et al., 2006; DeMorrow, 2009; Mancino et al., 2009). It has also been reported that estrogen positively modulates cholangiocyte proliferation, which is associated with cholangiopathies found in humans
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Also important in binding of E
2020-01-21
Also important in binding of E2 to the ER is the hydroxyl on the D ring, which has a stabilizing contact with His-524 in ERα (Fig. 7) and with His-475 in ERβ (Fig. S1) [8,9,36,37]. The spatial relationship of the functional groups on the A and D rings on E2 has been used to develop chemicals contai
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The role of ARA cyp metabolites in
2020-01-20
The role of ARA-cyp450 metabolites in the liver is still unclear [44]. However, ARA-cyp450 genes metabolize other fatty acids such lauric and palmitic EZ Cap Reagent AG mg [45]. It was found that decreased cyp4a expression caused fatty livers through reduction of fatty acid metabolism [46]. In this
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In conclusion our work provides the preclinical evidence sho
2020-01-20
In conclusion, our work provides the preclinical evidence showing the potent in vivo efficacy of AT7519 on chemoresistant cancer D-NMAPPD australia and its underlying mechanisms of action. Our work supports the biological rationale behind the clinical trials initiated with AT7519, particularly for
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energy metabolism The present study manipulated dopaminergic
2020-01-20
The present study “manipulated” dopaminergic functioning by examining genetic variation related to the catechol-O-methyltransferase (COMT) gene. The gene transcribes the COMT enzyme involved in degradation of released dopamine in the frontal cortex. COMT is primarily localized to the frontal cortex