br Acknowledgements We are very grateful to

Acknowledgements We are very grateful to Lindsay Cowell and Albert Goldfain for assistance with designing BCIDO. This work was conducted using the Protégé resource, which is supported by Grant GM10331601 from the National Institute of General Medical Sciences of the United States National Institutes of Health. This research was partially supported by National Library of Medicine Grant R01LM009886 to Dr. Weng. Dr. Gordon is supported by a Hutchins Family Fellowship, Fulbright Postgraduate Scholarship and Australian American Association Fellowship. The article׳s contents are solely the responsibility of the authors and do not necessarily represent the official view of NIH.
Data
Experimental design, materials and methods
Acknowledgments This work has been supported by funding from the Spencer Foundation, Grant no. 201500153.
Specifications Table
Value of the data
Data Passive drip stains were developed from blood droplets of fresh porcine blood, blood thoroughly admixed with different dosage of Warfarin (i.e. 2mg, 4mg, 6mg, 8mg, 10mg) and Heparin (i.e. 260I.U., 520I.U., 780I.U., 1040I.U., 1300I.U.) emanated from a subcutaneous syringe with and without needle by varying the angle of impact in the range of 30°, 60° and 90° against fall height of 20, 40 and 60cm [1,2] respectively. The drip stains were photographed based on the guidelines followed for documentation of physical evidence in forensic science. Table 1 documents the standards used for archiving the drip stain pattern images.
Experimental design, materials and methods Pig blood was used in developing the bloodstain dataset as pig blood rheology closely resembles human blood rheology [3,4]. The experiment was undertaken at the Kolkata Municipal Pig Slaughter House with due permission from the concerned authorities. All experiments that PJ34 were conducted with fresh porcine blood were performed within 15min from the time of collection of blood from a freshly slaughtered pig. For experiments in which blood thoroughly admixed with Warfarin was used, the container in which blood was collected was lined with aqueous solution of Warfarin prepared with distilled water. Again, experiments in which blood thoroughly admixed with Heparin was used, the container in which blood was collected was lined with different dosage of Heparin based on experimental requirements. Blood admixed with Warfarin or Heparin was allowed to stand for 24h in an antechamber at the slaughter house Recessive alleles was maintained at 4°C with stirring by an automatic overhead stirrer at 20rpm [5]. Two hours prior to experimentation, blood admixed with Warfarin or Heparin was placed in a chamber maintained at 37°C with light stirring (20rpm) by an automated overhead stirrer. Experimentation was started only when the temperature of blood was found to be 37°C on a digital thermometer. The experiment was performed in 2 parts. The first part dealt with development of drip stains from blood drops emanated from a subcutaneous syringe with needle. The angle of impact and the fall height were varied. Fig. 1 represents the experimental setup. The subcutaneous syringe was clamped and a protractor was attached to the clamp as demonstrated in Fig. 1. In order to achieve a particular angle of impact, the angle of inclination of the target surface was varied. A centimeter scale was used to calibrate fall height. Pressure required to eject a single blood droplet pendant from the subcutaneous syringe with needle was empirically calculated. Table 2 represents the angle of impact and fall height combination set used for the development of drip stain with fresh porcine blood, 250ml of blood thoroughly admixed with different dosage of Warfarin and 250ml of blood thoroughly admixed with different dosage of Heparin. In the second part of the experiment, the experimental setup illustrated in Fig. 1 was used to develop drip stain patterns at varying angle of impact and fall height by allowing blood pendant formation and subsequent dripping under the action of gravity from a subcutaneous syringe with and without needle. Table 3 documents the specification of impact angle and fall height used for development of drip stain patterns in the second part of the experiment.