Archives
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br Materials and methods br Results br Discussion The glucos
2021-09-28
Materials and methods Results Discussion The glucose transporter GLUT1 can be acutely activated or inhibited in L929 fibroblast 1400w by a wide variety of reagents or nutrient conditions [[9], [10], [11], [12],36,50]. This abrupt change in the activity of GLUT1 occurs within minutes and wi
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cdk1 mg Based on the collective experience to date it
2021-09-27
Based on the collective experience to date, it is likely that a much more comprehensive assessment of GSI selectivity for inhibition of multiple substrates, as well as biomarkers to track inhibition of non-APP substrates in vivo will be needed to support future efforts to develop substrate selective
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Firefly Luciferase mRNA Ionotropic aminobutyric acid GABA re
2021-09-27
Ionotropic γ-aminobutyric Firefly Luciferase mRNA (GABA) receptors (GABARs) belong to the Cys-loop ligand-gated ion channel (LGIC) superfamily and play an important role in the rapid inhibitory synaptic transmission in nervous systems of both vertebrate and invertebrate (Buckingham et al., 2005). I
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NMDARs are glutamate gated ionotropic receptors that are inv
2021-09-27
NMDARs are glutamate-gated ionotropic receptors that are involved in excitatory neurotransmission and plasticity (Traynelis et al., 2010). There have been several reports showing that neurosteroids acting as negative modulators of NMDARs exert neuroprotective activity in both in vitro and in vivo mo
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Plasmid construction and site directed
2021-09-27
Plasmid construction and site-directed mutagenesis of M. luteus B-P 26 UPS. For the expression of N-terminus His6-tagged M. luteus B-P 26 UPS, the expression plasmid pMluUEX [7] was cleaved with the restriction enzymes NdeI/BamHI. The gene fragment was inserted into an NdeI/BamHI-treated pET-15b to
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br Introduction Stroke is the second cause of
2021-09-27
Introduction Stroke is the second cause of death worldwide and the first cause of acquired motor disability in adults [1], [2]. In sub-Saharan Africa, stroke is the first cause of admission in neurology departments [3]. In Cameroon, the in-hospital mortality of stroke is estimated at 26.8% [4]. A
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The histamine H receptor H R amino acids is located
2021-09-27
The histamine H3 receptor (H3R; 326–445 amino-acids) is located on histaminergic neuron somata, dendrites and axon varicosities, as well as on the axon varicosities and somata of other neurons, providing negative feedback to inhibit histamine synthesis and the release of histamine or other transmitt
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Sonidegib is currently under investigation
2021-09-27
Sonidegib is currently under investigation for the treatment of relapsed/refractory acute leukemia (NCT01826214) [76]. All patients experienced at least 1 AE, including anemia, diarrhea, fatigue, nausea, muscle spasms, decreased appetite, and blood creatine phosphokinase increased. The ongoing phas
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Biological evaluations of the compounds were done
2021-09-27
Biological evaluations of the compounds were done both using HCV protease and replicon assays. Results are shown in . The protease inhibitory IC’s were determined using a FRET assay with HCV NS3/4A 1a protease domain. The replicon EC’s were determined using a replicon luciferase cell-based assay. Th
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Beckman Coulter s DxN VERIS Molecular
2021-09-27
Beckman Coulter’s DxN VERIS Molecular Diagnostics System (DxN VERIS System) is a fully automated system for the quantitative analysis of molecular targets, integrating sample introduction, nucleic phosphodiesterase inhibitors extraction, reaction set-up, real-time PCR amplification and detection usi
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The gsnor ko plants contain elevated amount of
2021-09-27
The gsnor-ko plants contain elevated amount of low and high molecular weight S-nitrosothiols (SNO) indicating that GSNOR activity controls the level of both GSNO and indirectly protein-SNOs [13], [17], [18]. GSNOR deficiency has been shown to cause pleiotropic plant growth defects, impaired plant di
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Using embelin and its analogs as surrogate GPR agonists we
2021-09-27
Using embelin and its analogs as surrogate GPR84 agonists we discovered that GPR84 couples to G12 and G13 signaling pathways in addition to Gi, linking receptor function to Rho/Rac signaling and modulation of the cytoskeleton. In primary human macrophages, GPR84 activation leads to Gβγ signaling, Er
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br Experimental br Acknowledgments The
2021-09-27
Experimental Acknowledgments The authors are grateful to Fundamental Scientific Research Fund of Chinese Academy of Medical Sciences (No. 2016ZX350030) for financial support. Introduction 2-Lysophosphatidylcholines (1-acyl-glycero-3-phosphocholines, 2-LPC) are blood derived factors involve
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In this study three potential Otx binding sites
2021-09-27
In this study, three potential Otx-2 HOAt were found in the promoter region of the sheep GnRH gene: −1786 bp to −1770 bp, −825 bp to −819 bp, and − 529 bp to −523 bp. Combined with the results of the double-luciferase assay, it could be inferred that the binding site of Otx-2 in the core promoter re
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Since the late s synthetic
2021-09-26
Since the late 1940s, synthetic GC (e.g. prednisone, prednisolone, dexamethasone) have been used extensively in the treatment of chronic inflammatory conditions such as rheumatoid arthritis and asthma, and for their immunosuppressant action in preventing organ rejection post transplantation. Cortico