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br Methods br Results br Discussion
2018-11-08
Methods Results Discussion Originally, human bone marrow-derived CD34+ adenosine receptor agonist were defined as hematopoietic stem/progenitor-rich cells, while endothelial progenitor cells (EPCs), which play an important role in post-natal angiogenesis/vasculogenesis in ischemic tissues,
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br Materials and methods br
2018-11-08
Materials and methods Verification and authentication Karyotyping was performed at the Center for Applied Human Molecular Genetics, Kennedy Center, Glostrup, Denmark. At least 10 metaphases were analyzed per sample with an approximate resolution of 550 to 600 bands per haploid genome. The resu
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Microarray based global hierarchical clustering clearly sepa
2018-11-08
Microarray-based global hierarchical clustering clearly separated all the directly isolated keratinocyte populations. In the first round of analysis () where we focused on known and well characterized populations the Plet population clearly separated from the rest of the populations, which is probab
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The karyotype of the BIOT LOAD
2018-11-08
The karyotype of the BIOT-4828-LOAD iPSC line was determined with Giemsa-banding, proving normal diploid 46, XX karyotype, without any detectable abnormalities (Fig. 1B). Expression of pluripotency markers was examined by immunocytochemistry staining, using pdgfr against human OCT3/4, E-CADHERIN, a
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br Conclusions The following are the supplementary
2018-11-08
Conclusions The following are the supplementary data relate to this article. Disclosure of potential conflicts of interest Acknowledgments We thank Fondazione Roma for continuous support. We also acknowledge Prof. Mara Riminucci, Prof. Luca Pierelli, Dr. C. Murdoch, Dr. Paola Iudicone and
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pterostilbene Introduction The stem cells extracted from the
2018-11-08
Introduction The stem cells extracted from the inner cell mass of an embryo are pluripotent, i.e. they have unique ability of long-term self-renewal and the potential to develop into all specialized cell types (Evans and Kaufman, 1981; Martin, 1981). However, embryonic stem cells with different ori
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The leading approaches to this are DA
2018-11-08
The leading approaches to this are DA cell transplantation, or stimulating endogenous DA neurogenesis, in either SNc or its efferent target the dorsal striatum. Despite progression of cell transplantation to clinical trials, it is still not widely available due to lack of a standardized and morally
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Safe Ca antagonists are already used clinically
2018-11-08
Safe Ca2+ antagonists are already used clinically, and convincing (albeit limited) evidence supports their use in LQTS diseases that resist conventional pharmacotherapy (Iseri and French, 1984; Jackman et al., 1990; Komiya et al., 2004). However, physicians appear hesitant to prescribe Ca2+ antagoni
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In addition to apoptosis we investigated the
2018-11-08
In addition to apoptosis, we investigated the occurrence of DNA lesions in response to genotoxic insults. Using LORD-Q analyses, a novel sensitive technique to quantify DNA lesions (Lehle et al., 2014), we found that, despite their elevated apoptosis sensitivity, iPSCs accumulated significantly less
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br Introduction In mammals cells from the blastocyst stage c
2018-11-08
Introduction In mammals, cells from the abacavir stage can be used to form embryonic stem (ES) cell lines that can self-renew in culture while maintaining the ability to differentiate into cells of the three germ layers (Reubinoff et al., 2000). Directed in vitro differentiation protocols general
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While we only implemented ArrayEdit
2018-11-08
While we only implemented ArrayEdit for fluorescence loss and growth rate differences, the method employing HCA is extendable in principle to any image-based phenotype. Phenotypes could be defined by changes in uptake of cytoxicity dyes, live immunocytochemistry for cell surface or extracellular mat
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Importantly because none of these markers are
2018-11-08
Importantly, because none of these markers are specific for trophoblast or trophoblast populations in general, they must be used in combination. For example, SWAN-71 and HTR8/SVneo, two widely used “trophoblast” cell lines, are hypermethylated at the ELF5 promoter, HTR8/SVneo does not express C19MC
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In this study we transplant hESC RPE to a large
2018-11-08
In this study, we transplant hESC-RPE to a large-eyed animal. Human and rabbit eyes are of similar size, the main advantage compared with previously studied mouse and rat models (Carido et al., 2014; Lund et al., 2006). Transplantation in rodents requires high cell concentrations (i.e., typically 5
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Our analysis revealed that mTOR inhibitors added alone to
2018-11-08
Our analysis revealed that mTOR inhibitors added alone to SF-defined medium can induce mesendoderm, and our follow-up studies confirmed that rapamycin enhances the formation of HE and blood progenitors. Ten-point dose curves revealed a bimodal effect where rapamycin at low concentrations mildly enha
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Using both standard RNA seq and
2018-11-08
Using both standard RNA-seq and RNA-seq Sulfo-NHS-Biotin analysis, we identified UPF1-regulated transcripts that are good candidates to be direct NMD targets in hESCs. There was considerable overlap between these candidate NMD target transcripts expressed in hESCs with previously identified high-co
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